After an evening in Louisville and dinner at Maker's Mark Bourbon House & Lounge, base yourself in Bardstown for the first night (the Jailer's Inn is a lovely historic B&B), and Frankfort the next (try the Meek House Bed & Breakfast). Hit Beam, Heaven Hill, and Maker's on your first day and Four Roses, Wild Turkey, and Buffalo Trace the next. Go during the week if you can, as there is far less to see on the weekends.
Jailer’s Inn Bed and Breakfast
Heaven Hill Bourbon Heritage Center
Jim Beam American Outpost
Anderson County, Kentucky
Franklin County, Kentucky
Here are the criteria that make Kentucky Straight Bourbon different from whiskey produced everywhere else (and it just so happens to be law in Kentucky).
It must contain 51 percent corn mash.
It must be distilled at no more than 160 proof.
It cannot go into the barrels at higher than 125 proof.
It must be aged for a minimum of two years in charred new white oak barrels.